Practical HPLC Training Program

About Liberannex & Scienceskool

This online HPLC course is proudly offered by LiberAnnex Analytical Services in collaboration with ScienceSkool, combining strong industry expertise with modern scientific education delivery. LiberAnnex Analytical Services is a specialized analytical research and testing organization focused on analytical method development, validation, stability studies, and advanced instrumental training in techniques such as HPLC, GC, and spectroscopy. With a team of experienced scientists and a commitment to industry-relevant skill development, LiberAnnex aims to bridge the gap between academic learning and practical laboratory applications.

ScienceSkool is a science learning platform dedicated to making high-quality technical education accessible through structured online programs, workshops, and skill-based training initiatives. By partnering together, LiberAnnex and ScienceSkool bring a powerful blend of real-world analytical experience, practical insights, and learner-friendly teaching methods to help students, researchers, and industry professionals build strong competence and confidence in HPLC.

About THe HPLC course- Skill-Based Training for Science & Pharmacy Students

High Performance Liquid Chromatography (HPLC) is one of the most important analytical techniques used in the pharmaceutical, biotechnology, food, and environmental industries. This short intensive training program is designed to provide students with both conceptual understanding and practical exposure to HPLC instrumentation and data analysis.

The course combines fundamental theory, instrument demonstration, chromatographic data interpretation, and troubleshooting knowledge, helping participants understand how HPLC is used in real analytical laboratories.

This training is ideal for B.Sc., M.Sc., B.Pharm, M.Pharm students and freshers preparing for pharmaceutical QC/QA careers.

Course Highlights

✔ Fundamentals of Chromatography
✔ Complete HPLC Instrumentation Understanding
✔ Real Instrument Demonstration
✔ Chromatogram Interpretation
✔ HPLC Applications in Pharma Industry
✔ Troubleshooting Common HPLC Problems

Course Structure (5 Sessions)

Session 1: Fundamentals of Chromatography & Introduction to HPLC

Participants will learn the basic principles of chromatographic separation and understand how HPLC works.

Topics Covered:

• Principle of chromatography
• Stationary phase vs mobile phase
• Introduction to HPLC
• Reverse Phase vs Normal Phase HPLC

Session 2: HPLC Instrumentation & Working with Practical demostration

This session explains the complete HPLC system and its components.

Participants will observe live instrument operation and chromatogram generation.

Topics Covered:

• Mobile phase reservoirs
• Pump and solvent delivery system
• Injector / Autosampler
• HPLC Column and stationary phase
• Detector (UV/PDA overview)
• Data acquisition system

Understanding the HPLC flow path:

Mobile Phase → Pump → Injector → Column → Detector → Data System

Session 3: HPLC Data Analysis

Data Analysis Concepts:

Identification & Quantification methods

• Chromatogram interpretation
• Retention time identification
• Peak area vs peak height
• Basic quantification concept

Session 4: HPLC Applications

Participants will understand how HPLC is used in real pharmaceutical laboratories and other various industries.

Applications Covered:

• Different types of assays and analysis in pharma
• Biopharma and Biomolecules
• Forensic science
• Food industries
• Food and environmental analysis

Session 5: HPLC Troubleshooting

Practical guidance will be provided on identifying and resolving common chromatographic problems.

Troubleshooting Topics:

Problems, causes and solutions for problems in various parts of HPLC

• Peak tailing
• Peak fronting
• High back pressure
• Baseline noise or drift
• No peak detection

Learning Outcomes

After completing the course, participants will be able to:

✔ Understand chromatographic separation principles
✔ Identify HPLC instrument components
✔ Understand the working process of HPLC
✔ Interpret chromatograms and analytical data
✔ Recognize common HPLC issues and troubleshooting methods

Who Should Attend?

This course is ideal for:

• B.Sc Chemistry / Life Science students
• M.Sc Analytical Chemistry students
• B.Pharm / M.Pharm students
• Fresh graduates preparing for Pharma QC jobs

Course Duration

4 Hours | 5 Sessions

Includes:

• Theory
• Instrument demonstration
• Data analysis discussion
• Interactive Q&A