LiberAnnex is a training and analytical research center that helps students build practical skills for their careers. We provide hands-on training on instruments like HPLC, GC, UV-Vis, and FTIR, so students can gain real lab experience beyond theory. Our programs are designed to make you industry-ready and open opportunities in QC, QA, R&D, and regulatory fields. With expert guidance, small batch sizes, and career-focused learning, we ensure every student learns with confidence. At LiberAnnex, we believe in “learning by doing” to prepare you for real industry challenges. Our goal is to help students gain confidence, develop job-ready skills, and bridge the gap between academics and industry needs.

High-Performance Liquid Chromatography (HPLC) is one of the most widely used analytical techniques in pharmaceuticals, food, biotechnology, and research. This 3-hour live online training is designed to give students, researchers, and professionals a strong foundation in HPLC principles, instrumentation, and real-time operation.

Day -1

8th January, 2026; 2:00 to 3:00 pm

HPLC basics & Instrumenation

• Introduction to Chromatography
• Principle of Separation
• HPLC parts
• Data output & Interpretation
• Solvent Reservoirs & Degassing
• Pumps (Isocratic vs. Gradient)
• Injector & Autosampler
• Columns (C18, C8, specialty types)
• Detectors (UV, PDA, Fluorescence, RI)
• Chromatogram Interpretation

Day -2

9th January, 2026; 2:00 to 3:00 pm

Live demo of HPLC & Live Practical

• Setting up HPLC system
• Step by step software learning
• Mobile phase preparation & degassing
• Sample injection & run
• Chromatogram generation & interpretation
• Caffeine analysis from beverages using HPLC
• RSD calculation , Data interpretation & quantification calculation

Day - 3

10th January, 2026; 2:00 to 3:00 pm

HPLC advanced concept

• Method Development Basics
• Troubleshooting in HPLC
• Applications of HPLC
• Q&A & Interactive Discussion

Why join?

✅ 3-Hour Intensive Training

✅ Live Q&A with Experts

✅ Hands-On Demonstration

✅ Certificate of Participation

✅ Boost for Job Readiness

Key Learning Outcomes

✔️ Fundamentals of HPLC & Separation Principles

✔️ HPLC Instrumentation

✔️ Pumps, Injector, Column, Detector

✔️ Mobile Phase & Gradient Techniques

✔️ Method Development & Validation Basics

✔️ Troubleshooting Common HPLC Issues

✔️ Real-Time Live Demonstration of HPLC Run

✔️ Industrial Applications in Pharma, Food & Biotech